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Build-a-BUG I: How to Clone a Gene

Saturday, Feb 1 10:00a

How to clone a gene ! This five session course will walk you through the various ways in which genes can be isolated and cloned into bacteria and yeast, providing you with a foundation on the techniques of synthetic biology. The lab project portion of the course will entail isolation and cloning of the gene encoding the Taq polymerase protein, and you will go home with the skills to make your own Taq polymerase – the enzyme at the heart of PCR.
Along the way you’ll learn how to isolate DNA, how to
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